Samples should be acetylated if they contain a very low concentration of cAMP or cGMP, or when greater sensitivity is required. The sensitivity with acetylation is approximately ten-fold greater than the non-acetylated format. If the levels of cyclic nucleotide in the samples are unknown, it is recommended to try running the assay in few non-precious samples without acetylation first in order to determine if this step is necessary.

Leave a comment