I bought your ADI-900-020A kit and I just realized that the manual indicates to avoid lipemic samples. My plasma samples are lipemic. Is there a way I can try measuring ET-1 concentration with your kit?

How lipid can potentially interfere in the assay has not specifically been tested, but you might want to consider delipidating your samples before taking any further steps with them. In the manual for our Arg8-Vasopressin ELISA kit, Prod. No. ADI-900-017, we provide a protocol for delipidation which can be tried out also in this case.
If samples are thought to be lipemic, the following procedure can be used to delipidate prior to extraction.
1. Prepare mixture of 40:60 butanol:diisopropyl ether. Vortex.
2. Add equal volume of butanol:diisopropyl ether to sample. Vortex.
3. Centrifuge at 8,000 x g for 5 minutes.
4. Remove top organic layer and discard. Measure aqueous layer and transfer to new tube.

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