HomeKnowledge BaseI am using the Direct 8-iso PGF2a ELISA kit. After I add the HCl to neutralize my samples, it is not reading in the range of pH 6-8. What should I do?
I am using the Direct 8-iso PGF2a ELISA kit. After I add the HCl to neutralize my samples, it is not reading in the range of pH 6-8. What should I do?
At this point, you have added HCl to NaOH which will yield NaCl + H2O. There is little buffering capacity allowing the pH to fluctuate quite easily. Simply bring the pH to the range of 6-8 and continue on with the procedure
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