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Urine is usually the sample of choice for 8-OHdG when looking at DNA damage; however, which sample may be best can depend on many factors including the specifics of your individual study. When a kit is validated for particular markers spiked samples are being looked at, and not samples from an actual study. Therefore, a literature search is recommended. Citations that describe their study parameters, levels of success, and expected levels of 8-OHdG will be much more valuable tools for you as you decide which sample to go with for your study.
As previously stated, urine does seem to be widely used for a number of diseases when looking at 8-OHdG. Urine can have varying levels of a maker present depending on how hydrated a patient may be so often researchers will find a way to normalize their data. Enzo offers a kit for normalizing urine samples (ADI-907-030A). Creatinine levels stay fairly consistent between different urine samples; so, it is strongly recommended that you use this or another means for normalizing your data. Serum can typically introduce a potential for matrix interference; this is the case with any kit and sample but is very important for serum as the matrix effect is typically eliminated by finding the optimal dilution for your serum samples. Saliva is also a possibility but you will need to find the best possible means for collection and sample handling as saliva can often have lower levels of a marker than other sample types. You can find several methods for saliva sample handling online and if you were to choose this method. It is recommended to try a couple of methods first to be sure you find what is optimal for your study.