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Collect whole blood in a covered test tube. If commercially available tubes are to be used, the researcher should use the red-topped tubes. These are available from Becton Dickinson (BD). BD’s trade name for the blood handling tubes is Vacutainer. After collection of the whole blood, allow the blood to clot by leaving it undisturbed for 4 hours at room temperature or 24 hours at 4 °C. Remove the clot by centrifuging at 1000-2000 x g for 10 minutes in a refrigerated centrifuge (do not use the centrifuge brake).
The resulting supernatant is designated serum. Following centrifugation, it is important to immediately transfer the liquid component (serum) into a clean polypropylene tube using a Pasteur pipette. The samples should be maintained at 2-8°C while handling. If the serum is not analyzed immediately, the serum should be apportioned into 0.5 ml aliquots, stored, and transported at –20°C or lower. It is important to avoid freeze-thaw cycles because this is detrimental to many serum components. Samples which are hemolyzed, icteric or lipemic can invalidate tests with the kit.