The product datasheet for the specific product includes information on the compound's solubility. Many compounds that are not directly soluble in water can be dissolved in alcohols, or solvents such as DMSO or DMF to create a concentrated stock solution. These stock solutions are often reasonably stable for storage, and can be diluted to the desired final treatment concentration with water or aqueous media at the time of use.
We strongly recommend using anhydrous solvents whenever working with a compound that is not water soluble. Solvents like DMSO are hygroscopic, and if the solvent has picked up water from the atmosphere, it can be problematic. Even very tiny amounts of water can significantly impact the limit of solubility of hydrophobic compounds.
We strongly recommend using anhydrous solvents whenever working with a compound that is not water soluble. Solvents like DMSO are hygroscopic, and if the solvent has picked up water from the atmosphere, it can be problematic. Even very tiny amounts of water can significantly impact the limit of solubility of hydrophobic compounds.
In the majority of cases, solubility in either ethanol or DMSO will be high enough to allow for a 1:500 to 1:1000 dilution with water or aqueous buffer to achieve the final treatment concentration desired.
Small amounts of residual organic solvent (for example 0.1% DMSO) do not normally affect biological systems, however we strongly recommend that solvent controls be run alongside all experiments as controls. For those compounds where these common techniques are not sufficient, the datasheets provide additional instructions specific to the particular compound in question. Please contact our technical support for more product-specific recommendations regarding reconstitution, dilution and storage of individual compounds.