Home Knowledge Base How long should I incubate my LEADING LIGHT® Wnt reporter cells after adding the luciferase substrate?
Date Published: July 18, 2023
Signals should be read 10 minutes after adding the luciferase substrate with a multichannel pipette. The signal is stable enough to add substrate to an entire plate before reading. More than 20-25 minutes and the signal will start to decrease.
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