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NUCLEAR-ID® red DNA stain ( ENZ-52406) is fairly stable. We do not have specifics on how long the dye would be retained in the cells. However, this dye intercalates in double-stranded DNA. As a result, similarly to any other DNA intercalating dye, it is quite toxic to cells. In our tests, we found that two hours post-staining, 75 % of cells remain viable, and six hours post-staining, only about 25 % of cells are viable. Therefore, this dye is essentially useful for end-point assay. Relative to other similar dyes, the advantage of the NUCLEAR-ID® dye would lie in the fact that it is excited with lower energy and it requires lower concentration, resulting intrinsically less toxic to cells, compared to others.