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Have You Published Using an Enzo Product?
EFLUXX-ID® Green and Gold Dyes in ENZ-51029 and ENZ-51030 kits are proprietary AM-esters. EFLUXX-ID® dye is a hydrophobic non-fluorescent compound that readily penetrates the cell membrane and is subsequently hydrolyzed inside of the cells by intracellular esterases. The resulting probe is a hydrophilic fluorescent dye that is trapped within the cell unless actively pumped out by an ABC transporter. The fluorescence signal of the dye generated within the cells thus depends upon the activity of the ABC transporters. The cells with highly active transporters would demonstrate lower fluorescence because of the active efflux of the probe from the cell. With specific inhibitors of the various ABC transporter proteins, it is possible to differentiate between the three common types of pumps and identify the type of transporter active in the tgested cells.