How do we define Limit of Detection (LoD) and what is the percent reliability detection with AMPIPROBE® SARS-CoV-2 Assay Kit, ENZ-GEN215?

Date Published: July 18, 2023

The LoD for AMPIPROBE® SARS-CoV-2 Assay Kit (ENZ-GEN215 ) is defined from this study as the lowest concentration of SARS-CoV-2 that can be reliably detected at least 95% of the time.

Limit of Detection (LoD) of the AMPIPROBE® SARS-CoV-2 Test System for detection of SARS-CoV-2 N1 and N2 target

 

Replicate  Group No. SARS-CoV-2 RNA copies/mL No. of Samples Tested No. of Samples Positive Mean Ct Positivity %
N1 280 20 19 36.4 95
N2 280 20 20 35.1 100

 

 

 

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