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It is important to remove the Cell Growth Medium entirely before adding trypsin to the cells. Usually, clumping is caused by treating the cells with trypsin for too long. Your trypsin solution needs to contain EDTA. It will help with the trypsinization. I would suggest adding 2 to 3 mL of Trypsin-EDTA solution, previously warmed-up at 37°C, very delicately to the cells and incubating at 37°C for 1 to 3 minutes. In order to avoid clumping, it is important not to agitate or hit the flask while waiting for the cells to detach or to force the cells to disperse. Check the cells every minute during the incubation and if the cells are detached after one minute, you can proceed to the next step by gently re-suspending in 10 mL of Cell Growth Medium. After adding the Cell Growth medium to the cells that have been trypsinized, further gentle up-and-down pipetting with 1mL plastic tips can be envisaged before counting and splitting the cells 1:4 or greater into 100mm tissue culture plates.