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Have You Published Using an Enzo Product?
The easiest way to overcome sample matrix interference is by diluting your sample with assay buffer. In order to determine the minimum required dilution, take one sample and split it into two parts. To one of those parts, add a known amount of standard. Serially dilute both the spiked and unspiked samples with assay buffer (e.g. 1:2, 1:4, 1:8, 1:16, further if you wish) then test all of those samples in the assay. For each dilution determine the concentration and % recovery. The minimum required dilution is reached when % recovery is within 80% to 120% of the spiked analyte amount.
Sample dilution is the best option provided your samples have levels of analyte that remain within the analytical range of the assay after dilution. If that is not the case, extraction, as outlined in our kit insert, will be needed.