Have any of Enzo Live Cell Fluorescent probes been validated on 3D spheroid culture?

While we did not specifically tested our Live Cell Analysis products on 3D models, we do have a few application notes demonstrating the use of a few kits in this context. For example:
– Image-based analysis of a human neurosphere stem cell model for the evaluation of potential neurotoxicants (). Featured products (ROS-ID^{® Total ROS detection kit ENZ-51011, GFP-CERTIFIED® Apoptosis/Necrosis detection kit ENZ-51002, NUCLEAR-ID® Blue/Red cell viability reagent (GFP-CERTIFIED®) ENZ-5300.
– Use of 3D Cultured Human iPSC-Derived Hepatocytes for Long-Term Hepatotoxicity Studies (}). Featured products: ROS-ID^{® Total ROS/Superoxide detection kit ENZ-51010; MITO-ID® Membrane potential detection kit ENZ-51018; NUCLEAR-ID® Blue/Red cell viability reagent (GFP-CERTIFIED® ENZ-53005
– Inhibition of Hypoxic Tumor Cells using a Three-Dimensional Spheroid Model (}). Featured product: ROS-ID^{® Hypoxia/Oxidative stress detection kit ENZ-51042.
– Validation of a Novel Tumoroid-Based Cell Culture Model to Perform 3D in vitro Cell Signaling Analyses (}