Does the appearance of green aggregates under the microscope suggest increased mitochondrial potential with Mito-ID® Membrane potential detection kit, ENZ-51018?

The MITO-ID^® dye in the MITO-ID^® Membrane potential detection kit (ENZ-51018) is a dual-emission cationic dye to measure the mitochondrial membrane potential (MMP) in live cells. In energized or active cells, the MITO-ID^® Membrane Potential reagent rapidly accumulates as orange-fluorescent aggregates in the mitochondria due to their relative negative charge, while it exists as a green-fluorescent monomer in the cytosol. However, in cells with compromised MMP, the MITO-ID^® Membrane Potential reagent exists primarily as green-fluorescent monomers throughout the cytosol and no longer exhibits orange fluorescence in the mitochondria.

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