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This peptide should be re-suspended in methanol at a concentration of 10mg/ml to give a clear colorless solution. The following research article describes the use of this substrate in an enzymatic assay.
M. Rohrwild, et al. (1996) HslV-HslU: A novel ATP-dependent protease complex in Escherichia coli related to the eukaryotic proteasome. Proc. Natl. Acad. Sci. U. S. A. 93, 5808. PMID: 8650174
Release of AMC fluorescence can be monitored using fluorimetry (340-380nm excitation and 440-460 emission wavelengths).