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It is possible to kinetically monitor the ability of the cells to proliferate with CYTO-ID® Red long-term cell tracer kit (ENZ-51037) or CYTO-ID ®Green long-term cell tracer kit (ENZ-51036). Color choice depends upon the instrumentation available and whether or not the cells contain GFP. CYTO-ID®red long term cell tracer kit works well for GFP expressing cells. Cells would be pre-labeled with the dye and seeded onto the substrate. Cells could then be imaged or analyzed by FACS at various time points. Every time a cell divides, the fluorescent signal will decrease by half in the resulting daughter cells. During the 100 hour incubation period the cells actively divide. At least 4-5 population doublings occur in this period. As expected, after each population doubling, the fluorescence per cell decreases by half. Beyond 96-120 hours, the fluorescence is so low that we can no longer distinguish between the stained and unstained cell populations. Detailed protocols from suspension and adherent cells analyzed by microscopy of flow cytometry, can be found in the manual of these kits, available for download from the products webpage https://www.enzolifesciences.com/ENZ-51036/cyto-id-green-long-term-cell-tracer-kit/ and https://www.enzolifesciences.com/ENZ-51037/cyto-id-red-long-term-cell-tracer-kit/