Can the cells be trypsinized to determine protein aggregation in cell based assay for testing drug responsiveness?

Trypsin can be used with ProteoStat^® Aggresome detection kit.  Following lysis, detergent can be added to permeabilize the cells.  The larger aggregates remain insoluble and they can be sedimented by centrifugation and the smaller aggregates can be separated by ultracentrifugation. The detergent, membranes and soluble protein can be washed away and the Proteostat detection reagent can be used on the remaining proteins.

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