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The COX Activity kits measure activity alone, distinguishing between COX-1 or -2. In order to differentiate between the two enzymes, two COX inhibitors are included in each kit. Ibuprofen can be used as a non-specific inhibitor while NS-398 is COX-2 specific. The use of these two compounds will allow the researcher to determine how much activity is due to each COX enzyme in a given sample.