It is possible to use a dye which would not have spectral overlap with the EFLUXX-ID® dyes (ENZ-51029, ENZ-51030) and propidium dye. Since Propidium Iodide is used to identify dead cells in the assay, any other dye should not overlap with emission of the Propidium Iodide or the EFLUXX-ID® dye.
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