Can AMP’D® ELISA Signal Amplification Kit ENZ-KIT-100 be used with any commercially available ELISA kit to amplify signals with alkaline phosphatase (AP) substrates?

Date Published: July 18, 2023

ANS: AMP'D® ELISA Signal Amplification kit, ENZ-KIT-100 has a signal amplification substrate containing NADPH and enzyme system for increasing the sensitivity in any ELISA.  End user is required to optimize the incubation time as shown in the table on page 7 for their ELISA.

https://www.enzolifesciences.com/ENZ-KIT-100/ampd-elisa-signal-amplification-kit/

Signal amplification in sandwich ELISA

Total incubation time of
Amplification Reagents (min)

Approximate Fold Increase in Sensitivity

30 (15 for substrate,
15 for amplifier)

8

60 (30 for substrate,
30 for amplifier)

20

90 (45 for substrate,
45 for amplifier)

50

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