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- Reconstitute the lyophilized peptide with 0.1 mL distilled water or PBS. Be careful to reconstitute entire contents of vial as some product may be on the lid.
- To a separate tube, add appropriate amount of antibody.
- To that tube, add an equal weight of peptide per volume antibody (i.e., µg peptide per 1 µL antibody).
- Dilute the antibody/peptide solution to approximately 1 mL with Western blot blocking buffer and vortex well.
- Incubate at room temperature for 15-30 minutes.
- Use the antibody for procedures as you would normally use it.