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Target Validation

Find a Viable Candidate for Drug Development

Confirm the Therapeutic Potential of Your Drug Target

Target validation is a crucial stage in drug discovery, confirming the suitability of a specific biological molecule, like a protein or enzyme, for drug development. It begins with identifying potential targets through various methods, such as genetics and omics technologies. Functional studies, including in vitro or in vivo assays, assess the target’s role in disease processes. Biochemical assays and structural biology provide insights into the target’s activity and structure, while pharmacological profiling evaluates potential drug candidates for efficacy and safety.

Activity Assays

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Whether you are performing large-scale screens, or studying specific enzyme kinetics, our high activity enzymes, unique substrates, and convenient kits are designed to deliver high-quality hits.

High purity, high activity control enzymes
Novel substrate/developer chemistry
Reduce interference from candidate compounds

Modified Substrate-Based Format

FLUOR DE LYS® HDAC & Sirtuin Assays

Convenient Screening Formats from the Pioneer in Non-Radioactive Assays

For over a decade, the FLUOR DE LYS® deacetylase assay platform has revolutionized assay of HDAC & Sirtuin enzyme activity, freeing researchers from cumbersome protocols required with radiolabeled or other modified histone-based methods. Our high-quality assays utilize patented substrate/developer chemistry in combination with high-activity, high-purity enzymes, to deliver more high-quality hits. Broad-class HDAC/Sirtuin screening assays are available in chemiluminescent, fluorescent, and colorimetric formats.

Flexible HDAC & Sirtuin Assays

Non-radioactive, homogeneous, HTS compatible formats
Green & chemiluminescent assays available to minimize interference
Assay all HDAC classes from multiple sources
High-activity, in-house manufactured control enzymes
Widely cited in peer- reviewed literature

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Antibody-Based Format

Akt Pathway Discovery

From Target Selection to Lead Profiling

Akt/PKB has emerged as a central player in the signal transduction pathways activated in response to growth factors or insulin. It is thought to contribute to several cellular functions including cell growth, transcriptional regulation, cell survival and nutrient metabolism. It has been linked to a range of diseases including cancer and type II diabetes.

The Akt/PKB pathway is a popular target since it sits at the crossroads of both oncogenic and tumor suppressor signaling pathways. Enzo provides novel tools to help support cancer discovery from target selection, hit-to-lead discovery, and lead profiling.

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Imaging Assays

Illuminating your Results

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A distinctive combination of reagents comprised of exceptional fluorescent molecular probes including CELLESTIAL® dyes for live, fixed and permeabilized cells. Products for drug discovery and research, in dynamic intracellular analysis with high spatial and temporal resolution for target, pathway and cell signaling analysis, reactive oxygen and reactive nitrogen species (ROS/RNS) detection, apoptosis and cell viability, and organelle functional dynamics.

25% Off* CELLESTIAL® Cell-based Assays and Dyes

High Specificity, Exceptional Photostability, Clear Visualization, Great Savings*!

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Flow Cytometry Assays

All Flow Cytometry Assays

Flow cytometry allows researchers to measure several physical characteristics of cells including its shape, size, and internal complexity. It has long been used in cell counting and cell sorting but more recently, researchers have been using flow cytometry in a broad range of applications including: biomarker detection, cell signaling, pathway screening, protein engineering, and systems biology. Flow cytometry is inherently a robust technique that adapts easily to basic research, drug development, and diagnostics.

Enzo offers a variety of flow cytometry-based assays for cell viability, proliferation and death analysis, available in a wide range of formats to meet individual research needs. We also offer unique flow cytometry-based assays to measure oxidative stress and to profile multi-drug resistance. These flexible assays are compatible with all flow cytometry platforms, regardless of size or functionality. The assays are easy to perform and can be run quickly using small sample input, without sacrificing sensitivity.

Multiplexing Flow Cytometry

Flow cytometry is a powerful tool that enables single-cell analysis of expression of different targets. Multiplexing different fluorophores and probes allows the collection of meaningful information and complete cell characterization.

Multiplex, Real-time Analysis of Hypoxia and ROS

ROS-ID® Hypoxia/Oxidative stress detection kit

Detection of hypoxia and oxidative stress levels in cultured human HeLa. Cells were treated with hypoxia inducer (DFO) and ROS inducer (pyocyanin). Numbers in each quadrant reflects the percentage of cells. Results indicate that hypoxia and oxidative stress dye are specific

Cytokine Assays

All Cytokine ELISA Assays

Cytokines, including interleukins, interferons, and chemokines, are secreted signaling molecules that mediate and regulate immunity, inflammation and some developmental processes during embryogenesis. They also play an important role in angiogenesis and cancer. Cytokines can have pleiotropic, overlapping, and sometimes contradictory functions. This is dependent upon their concentration, the cell type they are acting on, and the presence of other cytokines and mediators. Enzo Life Sciences offers a wide selection of cytokine antibodies, proteins, and kits for innate and adaptive immune research including our wide selection of high-specificity human and mouse cytokine ELISA kits.

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Target Validation

Confirm the Therapeutic Potential of Your Drug Target

Activity Assays

Modified Substrate-Based Format

FLUOR DE LYS® HDAC & Sirtuin Assays

Antibody-Based Format

Akt Pathway Discovery

Imaging Assays

Illuminating your Results

25% Off* CELLESTIAL® Cell-based Assays and Dyes

Flow Cytometry Assays

Multiplexing Flow Cytometry

Multiplex, Real-time Analysis of Hypoxia and ROS

Cytokine Assays

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How are cell-based assays useful in drug discovery research?

What Assays are used for Drug Discovery & Development?

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