Cell Therapy

Cell therapies with T cells or stem cells hold the potential for treatments for a myriad of pathologies including cancer, cardiovascular, neurodegenerative diseases, among others. The integration of endogenous lymphocytes that can infiltrate tumors and the genetically modified T cells enhance the efficacy and applicability of cell therapy treatments for cancer or other diseases. Moreover, the unique properties of self-renewal and differentiation of stem cells offer novel therapeutic approaches for tissue repair and regeneration. Unlocking the cellular mechanisms of reprogramming and differentiation holds promise for new cellular therapies, regenerative medicine, and improving model systems to assess drug efficacy and toxicity.

Regardless of your cell therapy study path, we provide innovative tools to help you realize greater efficiency with our strong manufacturing capabilities coupled with our supply base. For over 45 years, Enzo has been collaborating with researchers worldwide, offering unique solutions to advance cell therapy research and accelerate the development and manufacturing of next-generation CAR-T and stem cell therapies. With our comprehensive catalog of unique products, we are committed to providing the tools and support you need to drive innovation and advancement in groundbreaking discovery in personalized medicine.

Monoclonal Antibodies for Flow Cytometry

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T Cells Gene Modification and Activation

Chimeric antigen receptors (CARs) are engineered T cell receptors to give T cells the ability to target a specifc antigen. T cell activation is a critical step for the expression of CAR T cells, wich can be done in the presence of activated B cells, acting as antigen presenting cells (APCs). One of the main challenges is maintaining an adequate level of B cell stimulation to activate the T cells.

T Cells Expansion

In many cases, the number of target T cells in peripheral blood is relatively low. The key at this phase is to develop efficient methods for stimulating the expansion of these cells ex vivo to obtain a sufficient quantity for therapy.
Enzo offer a wide range of solutions to scale-up T-cell expansion with our quality, highly tested and cited cytokines and growth factors.

T cell exhaustion arise from overstimulation, resulting in dysfunctional cells. Exhausted T cells lose their ability to respond effectively to antigens with significant implications for immunotherapy efficacy. Exhausted T cells often upregulate immune checkpoint receptors, such as PD-1 (programmed cell death protein 1) and Lymphocyte-Activation Gene 3 (LAG-3), and exhibit reduced pro-inflammatory cytokines production such as interferon-gamma, (IFN-γ) and interleukin-2 (IL-2), impacting cytotoxicity, and proliferative capacity.

Stem Cells Gene Editing and Expansion

Stem cells can be genetically engineered to express edited target genes, correcting disease-causing mutations. This gene modification process enhances stem cell therapies’ regenerative or immunomodulatory properties. The challenge at this stage is to have a controlled replication of stem cells in vitro to increase their numbers while maintaining their undifferentiated and self-renewal capabilities. Small molecules and growth factors play a pivotal role in regulating stem cell proliferation and maintaining their pluripotency or multipotency.

Stem Cells Differentiation

Stem cell self-renewal and differentiation involve complex events that lead to the generation of various progeny cells and tissues. The efficiency of cell differentiation is essential to achieving full cell maturation and functionality. Optimizing solutions to enhance the efficiency and scale-up of differentiation is an ongoing challenge.

Enzo offers an extensive collection of small molecules that can modulate differentiation, biochemical activity, and cell growth.

Cryopreservation and Characterization

Cryopreservation and characterization are the last stages of developing T cell and stem cell therapies. Preserving cellular viability, unctionality, and genetic stability during long-term storage is critical and challenging. Identifying any delayed or cumulative effects of cryopreservation-related toxicity is also essential.

Enzo has a long and successful track record in identifying, synthesizing, and commercializing valuable, known small molecules to efficiently accelerate the development and scale up the production of cell-based therapies.

At the end of the manufacturing process, it is essential to assess whether cell viability has been preserved during freezing and thawing.

Enzo fluorescent probe-based CELLESTIAL® assays enable multiplex characterization of cell populations, including real-time functional assays to assess cell viability, cell death, and toxicity.

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We are committed to supporting the growth of our customers, and helping them reach their goals.
Whether you are analyzing nucleic acids, peptides, proteins, antibodies, cells, tissues, or small molecules, our scientific team is here to support you from experimental design to data analysis. Our ISO 9001-certified/ISO 13485-certified and GMP US-based manufacturing facility ensures the highest standards in all Enzo products and services.
As scientists enabling scientists, Enzo’s dedicated technical teams are your partners every step of the way. We enable our customers to achieve their goals faster, more efficiently, and more effectively – creating full solutions that transform basic research into innovative drug discovery and target detection.