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Immuno histochemistry (IHC)
Eliminate Data Ambiguity
High Sensitivity. Low Background. True Results.
For decades, immunohistochemistry has been an invaluable tool for the detection, localization, and quantification of antigens in preserved tissue for research and diagnostic purposes. Enzo’s complete set of tools improve the quality and efficiency of your IHC process from start to finish. Avoid ambiguity with high-sensitivity and low background reagents that are compatible with manual and automated platforms. Combined with the market’s most extensive palette of unique chromogens, Enzo provides the flexibility needed for simple or complex protocols.
The POLYVIEW® PLUS HRP-DAB (Anti-Rabbit) kit worked very well; there was no background and the protocol is faster than many other IHC kits available commercially. I would surely recommend it. I was really happy with the POLYVIEW® PLUS HRP-DAB (Anti-Mouse) kit too.
Columbia University
We tested Enzo’s POLYVIEW® IHC detection reagents and HIGHDEF® chromogens on our immunohistochemical autostainers against similar available detection reagents and chromogens. We found that Enzo’s detection reagents and chromogens matched or exceeded what we currently used, allowing for increased sensitivity and specificity when used against both human and murine tissue.
The Beatson Institute
Enzo’s IHC Platform
Eliminates false positive results
Compatible with both manual and automated platforms
Consistent, reliable, and sharp results
Cost-effective and time-saving
Immunohistochemistry Application Notes
Complete Kits & Flexible Reagents for Immunohistochemistry
TISSUE MICROARRAY
- Diverse set of 11 tissues
- Reactivity for over 130
biomarkers - Rapid, inexpensive
screening tools
MULTIPLEX IMMUNOHISTOCHEMISTRY
- Conserve precious samples
- Reduce reagent costs
- Convenient kit format
DEVELOP
HIGHDEF® Brown, Blue, Black, Red & Yellow HRP Chromogens
HIGHDEF® Red, Blue, & Green AP Chromogens
HIGHDEF® Hematoxylin
SINGLE COLOR IHC
- High sensitivity, low background
detection - Vibrant, unique chromogens
- Ancillary reagents
PRESERVE
Do you want to know more about IHC?
Download our Immunohistorchemistry E-book
Enzo offers the broadest (and expanding) palette of IHC stain colors to choose from, giving you the ultimate flexibility in planning your multiplex Immunohistochemistry protocol.
- Widest panel of color choices
- Unique yellow, black, and blue HRP chromogen
- Increased flexibility with HRP & AP options
- High definition color development
Formalin-fixed paraffin-embedded human tonsil stained with pre-diluted mouse CD68 antibody labeled with HIGHDEF® IHC chromogen substrate (DAB, HC) ( brown), pre-diluted rabbit lambda light-chain antibody labeled with HIGHDEF® blue IHC chromogen (AP) (blue), pre-diluted rabbit kappa light-chain antibody labeled with HIGHDEF® red IHC chromogen (AP, plus) ( red), high molecular weight cytokeratin labeled with HIGHDEF® yellow IHC chromogen (HRP) (yellow), and methyl green nuclear counterstain.
Discover the Immunohistochemistry Kit matching your Application
IHC Solutions
High Sensitivity. Low Background. True Results.
Application Note
Antibody Search
Our growing list of over 1000 IHC validated antibodies includes those for the detection of key signaling proteins, cell surface markers, mediators of cell death, oxidative stress, heat shock proteins, proteasomes, and more. We know IHC optimization can be a time and resource consuming endeavor. You can back any of our antibodies with our Worry-Free Guarantee, even those not validated for IHC.
Key IHC Validated Antibodies
Enabling Flexible Multiomics Analysis
Simultaneously analyze multiple targets, including nucleic acids, proteins, and organelles
No need to go anywhere else for the ancillary reagents and materials you need for your IHC process. Enzo offers a broad selection of components to enhance your data and simplify your ordering process at the same time.
Spatial Biology
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