qPCR and RT-qPCR

AMPIGENE® qPCR/RT-qPCR Solutions

AMPIGENE® qPCR Mixes use the latest developments in buffer chemistry to deliver high-performing dye-based or probe-based real-time PCR with added enhancers and stabilizers to improve reaction sensitivity and specificity. Available with different concentrations of the ROX reference dye (Hi ROX, Lo ROX, or No ROX)

AMPIGENE® qPCR Green Mixes

  • Proprietary intercalating dye that does not interfere with PCR
  • Antibody-mediated hot start PCR
  • Compatible on all standard and fast cycling real-time PCR platforms

AMPIGENE® qPCR Probe Mixes

  • Designed for use on a wide range of probe technologies (Taqman®, Molecular Beacons®, and Scorpion® probes)
  • Enhancers and stabilizers improve reaction sensitivity and specificity
Superior Performance at Lower RNA Concentration
Performance of AMPIGENE® qPCR 1-Step Green Kit vs.
Competitor. Amplification of increasing concentation of mouse liver RNA (25pg to 250ng per 20μl reaction) with actg1 gene primers.
Efficient Amplification of Total RNA Over a Broad Dynamic Range
Performance of AMPIGENE® qPCR 1-Step Probe Kit. Amplification of increasing concentation of mouse liver RNA (10pg to 1μg per 20μl reaction) with actg1 gene primers.

AMPIGENE® RT-qPCR 1-Step Kits

Provide optimized and efficient cDNA synthesis and qPCR in a single tube

AMPIGENE® qPCR 1-Step Kits (Dye or Probe-based) use the latest developments in reverse transcriptase (RTase) technology and buffer chemistry for efficient cDNA synthesis and qPCR in a single tube. The modified MMLV RTase is blended with an RNase inhibitor to prevent RNA degradation by contaminating RNase.

Product Information
    • Convenient one-tube reaction
    • Thermostable and extremely active MMLV RTase 
    • Good for detection of low copy number targets (1pg – 10ng total RNA or >0.01pg mRNA) 

AMPIGENE® qPCR 1-Step Green Kit Lo-ROX

ENZ-NUC108

Optimized and efficient cDNA synthesis and dye-based qPCR in a single tube

Application PCR

AMPIGENE® qPCR 1-Step Green Kit Hi-ROX

ENZ-NUC109

Optimized and efficient cDNA synthesis and dye-based qPCR in a single tube

Application PCR

AMPIGENE® qPCR 1-Step Probe Kit Lo-ROX

ENZ-NUC110

Optimized and efficient cDNA synthesis and probe-based qPCR in a single tube

1 citation

Application PCR

AMPIGENE® qPCR 1-Step Probe Kit Hi-ROX

ENZ-NUC111

Optimized and efficient cDNA synthesis and probe-based qPCR in a single tube

Application PCR