Isothermal DNA Amplification

AMPIGENE® Isothermal Amplification – Bst Polymerases and Mixes

AMPIGENE® Bst Polymerase (recombinant fragment of G.stearothermophilus DNA polymerase) is ideal for nucleic acid amplification methods taking place at a single incubation temperature — such as whole genome, multiple displacement, and isothermal amplifications, including loop-mediated isothermal amplification (LAMP).

  • Operates over a broad temperature range
  • Rapid and consistent results
  • Flexible format – available with or without dye (for real-time detection with any thermocycler or combination heat source and detector)
Complete Product Range
Isothermal amplification of SARS-CoV-2 N gene target using AMPIGENE® Bst 1-Step Mix  in comparison to a competitor’s product.
The primer mix primers target a region of the viral N gene and were used at the following concentration: 1.6 μM FIP, 1.6 μM BIP, 0.2 μM F3, 0.2 μM B3, 0.8 μM Loop F, 0.8 μM Loop B. Bst 1-Step Mix displayed earlier times to result and higher sensitivity compared to the competitor’s product. Amplification of 13 copies of SARS-CoV-2 genome was obtained within 30 min.

AMPIGENE® Lyo-Probe Kits

AMPIGENE® Lyo-Probe is a lyophilizable RT-qPCR kit for the sensitive detection of RNA sequences. This formulated kit is ideal for assay development to provide enhanced functionality without loss of activity post lyophilization.

Complete Product Range
  • Detection of low copy number targets
  • Antibody-mediated hot start technology with improved tolerance to common PCR inhibitors found in clinical samples
  • Glycerol-free buffer

AMPIGENE® PCR Solutions

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