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Endpoint PCR

AMPIGENE® End-Point PCR

PCR is a routine procedure, amplifying specific targets can still be challenging. Enzo has developed Taq DNA polymerase products to help you achieve your PCR results. And we offer these in pre-optimized mixes or as standalone enzymes.

What PCR product would be best for your application?

	AMPIGENE® Taq DNA Polymerase	AMPIGENE® Hot Start Taq DNA Polymerase	AMPIGENE® High Fidelity Polymerase	AMPIGENE® Hot Start High Fidelity Polymerase
*Application*
Standard PCR	Yes	Yes	Yes
Long PCR			Yes	Yes
High-throughput		Yes		Yes
Multiplex PCR		Yes		Yes
High fidelity PCR			Yes	Yes
PCR from solid tissue		Yes
GC-rich templates		Yes	Yes	Yes
Genotyping		Yes
Bisulfite PCR	Yes	Yes
Methylated DNA		Yes	Yes	Yes
TA cloning	Yes	Yes
Blunt end cloning	Yes		Yes	Yes
Colony PCR		Yes
Crude sample PCR		Yes
Site-directed mutagenesis			Yes	Yes
NGS			Yes	Yes
*Properties*
Amplicon length	<6 kb	<6 kb	<20 kb	<20 kb
Hot Start		Yes		Yes
Fidelity vs. Taq	1x	1x	100x	100x
3′ -> 5′ exonuclease (proofreading) activity			Yes	Yes
Prevents primer dimers		Yes		Yes
Processivity		17 nuc/sec	30-100 nuc/sec	30-100 nuc/sec
Amplification of GC and AT-rich templates to over 17.5kb			Yes	Yes
*Format Options*
Loading dye added			Yes	Yes
Ready mix	Yes	Yes	Yes	Yes

**AMPIGENE® Hot Start Taq DNA Polymerase for enhanced speed, yield, and sensitivity**

AMPIGENE® Hot Start Taq DNA Polymerase (ENZ-NUC123/ENZ-NUC125) uses the latest polymerase technology and buffer chemistry developments to improve PCR performance for difficult template targets. Our enzyme and buffer system allow for superior amplification from GC and AT-rich sequences.

Ultra-sensitive and reliable technology
Convenient ready-to-use 2x mix option (ENZ-NUC125)
Excellent for low copy number template detection
High performance with complex templates such as GC and AT-rich sequences and mammalian genomic DNA

Product Information

Strong Signal Provides Clear Results

AMPIGENE® Hot Start Taq DNA Polymerase(ENZ-NUC123) vs. Competitor. Human Genomic DNA (5ng) template was targeted with four separate primer sets in 20µl PCR vol.