Contamination Control of Genomic DNA

Remove contaminating gDNA for more reliable data

Contaminating genomic DNA (gDNA) in RNA preparations can lead to incorrect quantification of RNA in RT-PCR or high DNA background in RNA sequencing. This is particularly an issue during the quantification of low-copy transcripts or the sequencing of small RNA samples. Removal of gDNA is often a necessity to prevent high DNA background in RNA sequencing. This protocol can be applied to RNA preparations before next-generation sequencing (NGS).

The gDNA removal kit is based on a recombinant heat-labile dsDNase (HL-dsDNase) to remove gDNA from RNA preparations. Through HL-dsDNase technology, contaminating genomic DNA is cleaved at ambient temperature and the enzyme is irreversibly inactivated upon increasing the temperature over 50° C.

The gDNA is removed from RNA preparations to levels below the detection limit of RT-qPCR and the inactivation is gentle enough to preserve both quality and quantity of all present RNA (Fig. 1A and B).

Get control of your contamination
Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity

PCR is a sensitive technique used in research and diagnostics. Taq polymerases, dNTP’s, buffer components, and primers/probes are frequently contaminated with E. coli DNA, which may lead to false positives if bacterial DNA is the target.

The PCR decontamination kit uses a dsDNase to remove contaminating DNA from mastermixes. The double-strand specific property allows decontamination in the presence of primers and probe. The dsDNase is irreversibly inactivated by heating to 60°C in the presence of DTT, ensuring that DNA template added to the mastermix after inactivation will not be digested. The mastermix can be used immediately after decontamination to run PCR reactions.

AMPIGENE® Taq polymerases have been treated to remove host DNA and additional clean-up is not necessary. The PCR decontamination kit is recommended for decontamination of primers or competitor’s PCR mixes.

Quick and simple kit for purifying DNA from PCR, gels, and labeling reactions for a variety of downstream applications. Silica membrane technology in a mini spin column format to deliver reliable DNA purification from PCR products, agarose gels, and labeling reactions.

Enzo’s Columns Perform Equivalently to Leading Competitor’s Columns Post-CGH Labeling