EQ Quencher

Dyes for Molecular Biology FRET Applications

EQ Quencher Dyes are Compatible With a Wide Range of Fluorescent Dyes

EQ quencher dyes are suitable as acceptors in fluorescence resonance energy transfer (FRET) applications due to their broad visible absorption but no detectable fluorescence emission.

  • Commonly used in molecular biology FRET applications to quench fluorophores
  • NHS ester quencher dye can be conjugated to amine-labeled probes
  • Enable multiplexing of probes with the use of different reporter-quencher dye pairs
  • Compatible with most common commercially available fluorophores including Alexa Fluor®, ATTO, DyLight®, and BD Horizon dyes
Normalized Absorbance of EQ Dyes

Normalized absorbance of EQ dyes showing their range of quenching through FRET.

EQ0 NHS ester

ENZ-CHM208

Amine-reactive quencher dye

Appearance Red brown solid.
Emission Maximum none
Excitation Maximum 445 nm
Purity ≥80% (HPLC)

EQ1 NHS ester

ENZ-CHM165

Amine-reactive quencher dye

Appearance Dark red solid.
Emission Maximum none
Excitation Maximum 535 nm
Purity ≥80% (HPLC)