Nucleic Acid Detection

The Power of in situ Hybridization (ISH) in Spatial Biology

Enzo’s spatial nucleic acid detection platform empowers researchers to visualize gene expression and genomic alterations within the native architecture of cells and tissues.

At the core of this offering are our AMPIVIEW® RNA Probes, powered by Enzo’s LoopRNA™ ISH technology to deliver superior sensitivity, flexibility and adaptability. This is a powerful, probe-based in situ hybridization system designed to detect specific DNA, DNA/RNA or RNA transcripts at the single-cell level with exceptional clarity and specificity.

Step 1 : Pre-Treatment

Step 2 : 2-Hour Hybridization

Chromogen Detection

Biotin-conjugated Probes

Hapten-conjugated Probes

Fluorescence Detection

Hapten-conjugated Probes

Hapten-conjugated Probes

Light Microscope

Fluorescent Microscope

Singleplex Detection of HER-2 in Breast Cancer Tissue

HER-2 detected in breast cancer tissue with A.-B. AMPIVIEW® HER-2 (AS) Dig RNA Probes and C.-D. with AMPIVIEW® NSP Dig RNA Probes (negative control probe). AMPIVIEW® RNA probes have been amplified with DIGX® rabbit anti-digoxigenin linker and detected with POLYVIEW® PLUS AP (anti-rabbit) reagent, combined with HIGHDEF® Red AP Chromogen Kit and counterstained with HIGHDEF® Hematoxylin.

Singleplex Detection of PD-1 in Ovarian and Prostate Cancer Tissues

PD-1 was fluorescently detected with AMPIVIEW® PD-1 (AS) Dig RNA Probes in A. ovarian cancer tissue (red signal) and B. prostate cancer tissue (green signal). DAPI was used as a nuclear marker.

Multiplex Nucleic Acid Targets in Different Types of FFPE Tissues

PD-1 (grey), PD-L1 (red) and EGFR (green) detected in A. Mantle lymphoma tissue, B. ovarian cancer tissue and C. prostate cancer tissue with AMPIVIEW® PD-1 (AS) Dig RNA Probes, AMPIVIEW® PD-L1 (AS) DNP RNA Probes and AMPIVIEW® EGFR (AS) Dig RNA Probes. DAPI (blue) was used as a nuclear marker

Multiplex Detection of Non-Coding RNAs and mRNAs in Brain Tissue

Detection of A. Nestin RNA (red), B. non-coding RNA activated by DNA damage (Norad) (green), C. circular RNA Circ018 (gray) and nuclear marker, DAPI, in rat brain tissue by hybridizing AMPIVIEW® RNA probes against Nestin, Norad and Circ018. Co-localization of Nestin and Norad indicates that the majority of the neural stem cells in the hippocampus express lncRNA Norad, but only a subset in the CA3 regions express Circ018. E. 4X magnification, F. 20X magnification.

AMPIVIEW® RNA Probes Achieve PCR-Level Single-Copy Sensitivity While Preserving Crucial Tissue Morphology

Single cell resolution further expands the understanding of gene expression in cell lines and tissue samples.

AMPIVIEW® RNA probes patented design enables highly specific and sensitive detection of target RNA, DNA or DNA/RNA, delivering robust, high-signal-to-noise performance at the single-molecule level with single-cell resolution. Powered by Enzo’s LoopRNA™ ISH technology, this powerful tool allows precise identification of genes and gene transcripts, deepening insights into gene expression, spatial localization within its morphological context.

Reliable Detection of Ubiquitin as Positive Control in Prostate Cancer Tissue

Detection of ubiquitin in prostate cancer tissue with A. C. AMPIVIEW® Ubiquitin (AS) Dig RNA probes (ENZ-GEN125) and B. D. Leading competitor’s probes. ISH and target detection performed according to manufacturer’s protocol.

Sharper Signal for EGFR and Cleaner Background with AMPIVIEW® RNA Probes in Prostate Tissue

Detection of EGFR in prostate cancer tissue with A. C. AMPIVIEW® EGFR (AS) Dig RNA probes (ENZ-GEN129) and B. D. Leading competitor’s probes. ISH and target detection performed according to manufacturer’s protocol.

Clean Background Matters: Negative Control Comparison of AMPIVIEW® RNA Probes vs Competition

Comparison of negative control probes in prostate cancer tissue with A. C. AMPIVIEW® NSP Dig RNA Probes and B. D. leading competitor’s negative control probes.