Matrix Metalloproteinase (MMP) Drug Discovery Kits

Redder MMP Substrates. Better Activity Assays.

Proteins of the matrix metalloproteinase (MMP) family are collectively involved in the breakdown of extracellular matrix proteins and processing of bioactive molecules and are required for some normal processes. Unfortunately, these proteins are also associated with many pathological processes including cirrhosis, arthritis, metastasis, and chronic cardiovascular diseases. Collagenases (Matrix Metalloproteinase-1, MMP-8, and MMP-13) and gelatinases (MMP-2 and MMP-9) also break down skin collagens, contributing to aging and wrinkling. Enzo offers high sensitivity colorimetric and fluorometric activity assays for screening collagenase and gelatinase inhibitors as well as highly active enzymes and specific antibodies for all your MMP research needs.

The MMP RED and GREEN Drug Discovery Kits are complete assay systems designed to screen MMP inhibitors, using long-wavelength quenched fluorogenic substrates. The assays are performed in a convenient 96-well microplate format, with the compound NNGH also included as a prototypic control inhibitor.

• Improved red-shifted substrate with better kinetics and brighter signal
• Includes active recombinant enzyme, substrate, and assay buffer
• Convenient real-time kinetics of cleavage is easily determined
• Microplate format for high-throughput screening

Avoid Interference with MMP Green and Red Substrates

Figure 1: Fluorescent emission spectra of small molecule drug camptothecin (light blue), OE33 cells (dark blue), MMP GREEN substrate (green), and MMP RED substrate (red), showing that the MMP GREEN and RED substrates avoid the interference caused by small molecules and cells.

Figure 2: Determination of k_cat/K_m for MMP-1 using OMNIMMP® RED. K_m = 0.48 µM,

Figure 3: Inhibition of ten MMPs by 1.3 µM NNGH.