Proteolysis & Protein Degradation

Tools for Monitoring Proteolysis & Protein Degradation

Over 500 proteases that have been identified in the genome comprise a diverse enzyme family critical for many vital processes. Lysosomal proteases (e.g. cathepsins) and the proteasome comprise the degradative pathways for protein turnover, misfolded, and damaged proteins, and thus are major contributors to protein homeostasis, i.e. proteostasis. In contrast, precise and limited proteolysis (e.g. MMPs, ADAMs, caspases, DPPIV) are increasingly recognized as an important form of regulation that activates or inactivates many signaling targets including other proteases, growth factors, and peptides.

Proteasome ELISA kit

Highly cited kit for proteasome quantification.
This kit provides the means to quantify 20S proteasome concentrations in biological samples using a sandwich ELISA technique, utilizing two 20S proteasome specific antibodies for capture and detection purposes together with a highly sensitive substrate. Sample 20S proteasome levels are determined by comparison to a 20S proteasome calibration curve produced in parallel.

Redder MMP Substrates. Better Activity Assays

The MMP RED and GREEN Drug Discovery Kits are complete assay systems designed to screen MMP inhibitors, using long-wavelength quenched fluorogenic substrates. The assays are performed in a convenient 96-well microplate format, with the compound NNGH [1] also included as a prototypic control inhibitor.

• Improved red-shifted substrate with better kinetics and brighter signal
• Includes active recombinant enzyme, substrate, and assay buffer
• Convenient real-time kinetics of cleavage is easily determined
• Microplate format for high-throughput screening