Metabolism
Advancing Metabolic Research for a Healthier Future

Tools for Advancing your Metabolic Research
Metabolism encompasses the intricate biochemical processes by which organisms convert substrates into energy, synthesize and repair cellular structures, and regulate physiological functions. Central to these processes are key biomolecules, including lipids, carbohydrates, proteins, and bioactive compounds such as vitamins and hormones. These molecules are integral to various metabolic pathways, including glycolysis, the tricarboxylic acid (TCA) cycle, and oxidative phosphorylation. Perturbations in these pathways can lead to pathological states such as diabetes mellitus, obesity, cancer, and neurodegenerative diseases. Understanding these complexities is pivotal for advancing therapeutic strategies and enhancing disease prevention, paving the way for a healthier future.

Enzo offers a range of solutions for investigating lipid metabolism, including specialized assay kits, bioactive molecules, and ELISA kits. These tools are designed to support researchers in advancing their understanding of lipid-related metabolic pathways and developing therapeutic strategies for metabolic diseases.
Adipogenesis Assay Kit
The Adipogenesis Assay Kit is designed for researchers studying adipocyte differentiation. This kit provides all necessary reagents for inducing and detecting adipogenesis in the classic 3T3-L1 cell model.
- Optimized Reagents: Includes differentiation, insulin, and enhancer solutions for maximal adipocyte induction.
- Colorimetric Detection: Adipogenesis Dye allows for visual confirmation and quantification using a spectrophotometer or microplate reader.
- Versatile Applications: Suitable for drug screening and testing agonists/antagonists of adipogenesis.
Reliable and Convenient Induction and Detection of Adipogenesis

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Carbohydrate metabolism comprises the complex biochemical processes involved in the catabolism and anabolism of carbohydrates to produce cellular energy. This metabolic framework includes key pathways such as glycolysis, the tricarboxylic acid (TCA) cycle, and gluconeogenesis. Regulatory hormones, including insulin and glucagon-like peptide-1 (GLP-1), play pivotal roles in modulating these metabolic pathways. Insulin facilitates cellular glucose uptake and glycogenesis, while GLP-1 potentiates insulin secretion in response to nutrient intake. Proper regulation of carbohydrate metabolism is essential for maintaining blood glucose levels. Dysregulation can lead to metabolic diseases such as diabetes mellitus and metabolic syndrome, highlighting its importance in understanding and managing these conditions.
ELISA Kits and Antibodies
Enzo’s expertise in producing high-quality products, including ELISA kits and antibodies, ensure that your research is supported by accurate and reproducible results.
- High Sensitivity and Specificity: Rigorously vetted to ensure precise detection of metabolic biomarkers, providing reliable and reproducible results.
- Peer-Reviewed Validation: Tested reliability and acceptance in the academic community through strong citation records.
- Focused Applications: Ideal for carbohydrate metabolism research, including insulin quantification and analysis of glucose regulatory pathways.
Enhanced Sensitivity and Performance of the Insulin ELISA Kit

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Protein metabolism, including the synthesis, breakdown, and recycling of proteins, is vital for maintaining physiological functions such as enzyme activity, cellular structure, and immune responses. The synthesis of proteins involves the assembly of amino acids into polypeptide chains, which are then folded into functional proteins. Conversely, the breakdown and recycling of proteins ensure the removal of damaged or unneeded proteins, allowing amino acids to be reused for new protein synthesis. This dynamic balance plays a significant role in regulating metabolic pathways, impacting health and disease, offering therapeutic potential, influencing nutritional strategies, and affecting aging.
Small Molecules in Protein Metabolism
Small molecules are invaluable tools in protein metabolism research, as they can modulate enzyme activity, influence protein-protein interactions, and affect signaling pathways. These compounds help researchers elucidate the mechanisms of protein function and regulation, providing unparalleled insights into metabolic processes.
- Extensive Collection: Over 3,000 stand-alone small molecules, including enzyme inhibitors, receptor ligands, and natural products.
- GMP Services: Enzo offers GMP manufacturing services, ensuring high-quality, compliant products for therapeutic development.
- High Purity Standards: All small molecules are rigorously tested for purity and consistency, ensuring reliable research outcomes.
Leupeptin
Leupeptin is a competitive and reversible protease inhibitor of serine and cysteine proteinases like calpain, trypsin, and papain. Leupeptin is also known to inhibit activation-induced programmed cell death, maintaining the viability and functionality of stem cells. By preventing protease activity, leupeptin helps researchers study protein stability, turnover, and the mechanisms underlying various diseases.

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Bioactive molecules such as hormones, secondary messengers, and vitamins play pivotal roles in regulating metabolic pathways and maintaining homeostasis. Hormones like insulin, cortisol, and thyroid hormones are essential for controlling energy balance, glucose metabolism, and overall health. Secondary messengers, including cyclic AMP (cAMP) and calcium ions, act as intermediaries in cellular signaling, amplifying and transmitting signals to ensure proper cellular responses. Vitamins, such as B vitamins and vitamin D, function as coenzymes and antioxidants, supporting various metabolic processes and preventing deficiencies that can lead to metabolic diseases. Investigating these molecules offers valuable insights into the complex network of metabolic regulation, leading to breakthroughs in disease prevention and management.
Direct cAMP ELISA kit
The Direct cAMP ELISA Kit is a highly sensitive colorimetric competitive immunoassay designed for the quantitative determination of intracellular cyclic AMP (cAMP) in cells or tissues. This kit utilizes a straightforward protocol with pre-coated plates and color-coded reagents, making it easy to use and reducing potential errors.
- Exceptional Sensitivity: Optional acetylation protocol increases sensitivity more than 10-fold, allowing detection as low as 0.006 pmol/mL.
- Rapid Assay Time: Complete results in just 3 hours, accommodating up to 39 samples in duplicate.
- Species Independent: Compatible with samples from any species, providing versatility for diverse research needs.
High Sensitivity Detection of cAMP

(Non-acetylated).
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Mitochondria are vital organelles within eukaryotic cells, primarily responsible for producing adenosine triphosphate (ATP) through the process of oxidative phosphorylation. This energy production is fundamental to numerous cellular functions and overall metabolic processes. The study of mitochondrial function is particularly relevant in metabolism research because it sheds light on how cells manage energy, maintain homeostasis, and respond to stress. Understanding mitochondrial dynamics, including their biogenesis and role in apoptosis, can reveal critical insights into metabolic diseases, aging, and potential therapeutic strategies for conditions like diabetes, neurodegenerative disorders, and cancer.
MITO-ID® Membrane Potential Detection kit
The MITO-ID® Membrane Potential Detection Kit is designed to measure mitochondrial membrane potential (MMP) in live cells using a dual-emission cationic dye. This kit is part of the CELLESTIAL® Cellular Analysis product line, which offers advanced tools for studying cellular functions and dynamics. The MITO-ID® kit provides a reliable, mix-and-read, no-wash protocol for assessing mitochondrial health and function.
- High Sensitivity: 10 times more sensitive than JC-1 dye, allowing for precise detection of changes in mitochondrial membrane potential.
- Versatile Applications: Suitable for fluorescence microscopy, flow cytometry, and microplate reader assays, making it ideal for various experimental setups.
- Rapid and Efficient: True mix-and-read homogeneous assay for live cells, streamlining the workflow and reducing potential errors.
MITO-ID® Membrane Potential Detection Kit for Microscopy and Flow Cytometry

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