FLUOR DE LYS® HDAC & Sirtuin Assays

Complete Toolbox for Non-Radioactive Assays

Convenient Screening Formats from the Pioneer in Non-Radioactive Assays

Flexible HDAC & Sirtuin Assays

For over decades, the FLUOR DE LYS® deacetylase assay platform has revolutionized assay of HDAC & Sirtuin enzyme activity, freeing researchers from cumbersome protocols required with radiolabeled or other modified histone-based methods. Our high-quality assays utilize patented substrate/developer chemistry in combination with high-activity, high-purity enzymes, to deliver more high-quality hits. Broad-class HDAC/Sirtuin screening assays are available in chemiluminescent, fluorescent, and colorimetric formats.

  • Non-radioactive, homogeneous, HTS compatible formats
  • Green & chemiluminescent assays available to minimize interference
  • Assay all HDAC classes from multiple sources
  • High-activity, in-house manufactured control enzymes
  • Widely cited in peer- reviewed literature


Stand-alone HDAC &
Sirtuin Substrates & Developers

FLUOR DE LYS® substrates form the core of the FLUOR DE LYS® Substrate/Developer system for measuring HDAC activity.

Featured FLUOR DE LYS® References

  • Yuan J, Luo K, Liu T, Lou Z. Regulation of SIRT1 activity by genotoxic stress. Genes Dev. 2012 Apr 15;26 (8):791-6.
  • Zhang S, Cai G, Fu B, Feng Z, Ding R, Bai X, Liu W, Zhuo L, Sun L, Liu F, Chen X. SIRT1 is required for the effects of rapamycin on high glucose-inducing mesangial cells senescence. Mech Ageing Dev. 2012 Jun;133 (6):387-400.
  • Wang Z, Li W, Meng X, Jia B. Resveratrol induces gastric cancer cell apoptosis via reactive oxygen species, but independent of sirtuin1. Clin Exp Pharmacol Physiol. 2012 Mar;39(3):227-32.
  • Vaidya AS, Karumudi B, Mendonca E, Madriaga A, Abdelkarim H, van Breemen RB, Petukhov PA. Design, synthesis, modeling, biological evaluation and photoaffinity labeling studies of novel series of photoreactive benzamide probes for histone deacetylase 2. Bioorg Med Chem Lett. 2012 Jun 18. [Epub ahead of print]