Monitoring Autophagy in Live Cells

Profile Autophagy Without Transfection

The CYTO-ID® Autophagy Detection Kit is a no-transfection quantitative assay for monitoring autophagy in live cells. Suitable for flow cytometry and microscopy, the assay facilitates high-throughput screening of activators and inhibitors of autophagy. The CYTO-ID® Autophagy Detection Kit includes a proprietary dye that selectively stains pre-autophagosomes, autophagosomes, and autolysosomes (autophagolysosomes), with minimal staining of lysosomes.

• Proprietary dye selectively stains autophagic vesicles, without time-consuming LC3-fusion protein transfection
• Rapidly quantifies autophagy in native heterogeneous cell populations
• Validated under a wide range of conditions and with small molecule modulators known to influence autophagy pathways
• Negligible staining of lysosomes reduces background seen with other dyes (e.g. MDC)

No Transfection

Starvation induces an increase in green fluorescence intensity as demonstrated by the presence of punctate cytoplasmic structures (top) compared to control cells (bottom). Nuclei counterstained with Hoechst dye (blue).