High Quality ELISAs for Mesuring Autophagy

Sensitive Kits for Detecting and Quantification

Sensitive p62 ELISA Kit allows for quantitative, immunometric detection of the autophagy biomarkers in human, rat and mouse cell lysates. The p62 protein has a dual functionality as both a scaffold protein and aiding in trafficking for protein degradation. ELISA kits enable quantitative measurement of autophagy without the need for expensive equipment or long procedures.

  • Sensitive assays measure as little as 100 pg/mL of p62
  • Fully quantitative results surpass semi-quantitative Western blot analysis
  • High throughput format allows analysis of up to 40 samples in duplicate in less than 3 hours
  • Easy-to-follow protocols and liquid color-coded reagents save time and reduce errors
Three Different Methods of p62 Detection and Quantification

Human breast cancer cells were treated with either autophagy-inducing drug or vehicle. Cells were harvested 6 and 12 hours post-treatment, lysed, and analyzed with the p62 ELISA kit, and for p62 and LC3- II by Western blot. Drug treatment correlated with the induction of autophagy as indicated by the decrease in p62 levels (A and B) and by elevation of LC3-II levels (C).

p62 ELISA kit

ADI-900-212

Highly validated, quantitative p62 ELISA kit available for autophagy research.

19 citations

Application Colorimetric detection, ELISA
Assay Time 3 hours
Sensitivity 100pg/ml  (range 625 – 40,000pg/ml)

Grp78/BiP ELISA kit

ADI-900-214

High-sensitivity ELISA kit for quantifying both normal and upregulated levels of GRP78/BiP for unfolded protein response, cancer and neurodegenerative disease research.

15 citations

Application Colorimetric detection, ELISA
Assay Time 2.5 hours
Sensitivity 8.4ng/ml (range 1.4-4500ng/ml)