Using Mouse-on-Mouse IHC Kits In Various Sample Types

INTRODUCTION

Accurate localization of mouse primary antibodies in mouse tissue is confounded by endogenous mouse immunoglobulins present in the tissue, which react with the anti-mouse secondary antibody reagent, causing off-target staining. In order to eliminate this background staining, reagents which block the endogenous mouse immunoglobulins and prevent non-specific staining are commonly used, and known as Mouse-on-Mouse or M.O.M. kits. As with any IHC reagent, multiple manufacturers field similar products which on their surface appear equivalent, but have widely varying performance characteristics. For this study, Enzo’s Mouse-on-Mouse blocking reagent was compared to two competitive products using 3 different primary antibody IHC reagents in 3 different mouse tissues. The primary antibodies were chosen to demonstrate staining in nucleus (phospho-Akt), cytoplasm (KDEL-R), and membrane (CD31).

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