Detecting Non-Coding RNAs with AMPIVIEW® ISH Probes

INTRODUCTION

Over the past decade, our understanding of the transcriptional landscape has undergone a dogma-challenging expansion, revealing the intricate role of nonprotein-coding RNAs (ncRNAs) in biological function, development, and disease.
Many newly discovered ncRNAs have been implicated in healthy cells’ molecular regulation and various cancers, cardiovascular diseases, and neurodegenerative disorders.
NcRNAs are structurally and functionally very heterogenous and include RNAs involved in post-transcriptional modification, DNA replication, and gene regulation. Regulatory ncRNAs have primarily been the focus of recent research efforts, amongst them notably microRNAs (miRNAs), which have emerged since their discovery in the early 1990s as a versatile cellular mechanism to modulate gene expression post-transcriptionally through silencing of messenger RNA (mRNA). These miRNAs typically contain 21-23 nucleotides,
distinguishing them from long non-coding RNAs (lncRNAs) with over 200 nucleotides. A third class of regulatory ncRNAs is circular RNA (circRNA) characterized by covalently closed continuous loops. Additionally, miRNAs, lncRNAs, and circRNAs are emerging as relevant contributors to cellular function in health and disease.

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