Jessica Antos1, Colten Wimmer2, Hartmut Pohl3
1Enzo Life Sciences, Ann Arbor, Michigan
2Byonoy GmbH, Hamburg, Germany
3Enzo Life Sciences, Lausen, Switzerland
INTRODUCTION
Since its introduction, Enzyme Linked Immunosorbent Assay (ELISA) has become a widespread and highly trusted tool to quantify a wide array of diverse analytes from small molecules to quaternary protein complexes. It has become a workhorse for a broad variety of research fields and is widely used for analytic and diagnostic purposes.
Laboratories and field-based research teams are hampered by the availability of affordable, portable, and low maintenance microplate reader instrumentation. This application note serves to analyze the performance of an ultra-compact microplate reader with established benchtop instrumentation.
To compare both instrument types, a variety of experimental setups have been performed in parallel, using both sandwich and competitive ELISA and the most common ELISA substrates for either type (TMB – tetramethylbenzidine and pNpp-p-Nitrophenylphosphate).