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Product Details
| Alternative Name |
β2 integrin |
|---|---|
| Application |
Flow Cytometry, FUNC, IHC (FS), IP, WB |
| Application Notes |
Functional Application: induces high-affinity LFA-1 conformation in leukocytes |
| Clone |
MEM-48 |
| Crossreactivity |
Cross-reacts with all three leukocyte integrins. |
| Formulation |
Liquid. In PBS, pH 7.4, containing 15mM sodium azide. |
| Host |
Mouse |
| Immunogen |
Leukocytes from a patient suffering from a LGL-type leukemia. |
| Isotype |
IgG1 |
| Purity Detail |
Protein A-affinity purified. |
| Recommendation Dilutions/Conditions |
Immunohistochemistry (paraffin sections: recommended dilution 10µg/ml, positive tissue: spleen, microglia; frozen sections)Western Blot (Recommended dilution: 2µg/ml in buffer containing laurylmaltoside, 2x non-reducing SDS; SDS-PAGE (6% separating gel, 4% stacking gel)positive control: Kg-1a human leukemia cell lysate)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. |
| Species Reactivity |
Human |
| Specificity |
Recognizes residues 534-546 in cysteine-rich repeat 3 of CD18 (β2 integrin). |
| UniProt ID |
P05107 |
| Worry-free Guarantee |
This antibody is covered by our Worry-Free Guarantee. |
Handling & Storage
| Handling |
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C. |
|---|---|
| Short Term Storage |
+4°C |
| Long Term Storage |
-20°C |
| Shipping |
Blue Ice |
| Regulatory Status |
RUO – Research Use Only |
|---|
- Regulation of CD18 expression on neutrophils in response to fluid shear stress: S. Fukuda and G.W. Schmid-Schonbein; PNAS 100, 13152 (2003), Abstract — Full Text
- A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins: K. Drbal, et al.; Immunobiology 203, 687 (2001), Abstract
- A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells: K. Drbal, et al.; Blood 98, 1561 (2001), Abstract
- Human leukocytes contain a large pool of free forms of CD18: K. Drbal; BBRC 275, 295 (2000), Abstract
- Monoclonal Lym-1 antibody-targeted lysis of B lymphoma cells by neutrophils. Evidence for two mechanisms of FcgammaRII-dependent cytolysis: L. Ottonello, et al.; J. Leukoc. Biol. 68, 662 (2000), Abstract
- Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins: L. Ottonello, et al.; Blood 93, 3505 (1999), Abstract — Full Text
- The nature of large noncovalent complexes containing glycosyl-phosphatidylinositol-anchored membrane glycoproteins and protein tyrosine kinases: T. Cinek and V. Horejsi; J. Immunol. 149, 2262 (1992), Abstract
- Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein: V. Bazil, et al.; Folia Biol. (Praha) 36, 41 (1990), Abstract
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