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Fas/APO-1/CD95 is a 36 kDa cell surface type I-membrane glycoprotein that is a member of the Tumor necrosis factor receptor (TNF-R) family, which includes TNF-R1, TNF-R2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis of Fas expressing cells. Apoptosis induced by Fas-Fas ligand interaction is inhibited by a number of cytosolic factors such as the ICE/CED3 family inhibitor CrmA, and the short and long form of FLIP.
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Western blot analysis of human Fas expression in human Fas transfected cells; Lane1: anti-human Fas (ZB4), Lane2: anti-human Fas (UB2) and Lane3: anti-human Fas (CH11).
Flow cytometric analysis of human Fas expression on normal human lymphocytes. Open histogram indicates the reaction of isotypic control to the cells. Shaded histogram indicates the reaction of Fas mAb (ZB4) to the cells.
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RUO – Research Use Only |
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