Western Blot

Simplify Your Western Blot: Six Simple Steps

Western blotting is viewed as the gold standard for protein detection in molecular biology research. It is used to identify proteins within a cell or tissue lysate. Antibodies against your protein(s) of interest, bind to specific epitopes to identify the target protein within a lysate. Due to the high specificity of the binding, multiple target proteins can be identified on one membrane. Secondary antibodies then bind to your primary antibodies and when exposed to a substrate react, allowing for the visualization of the corresponding protein band.

The WESTERNVIEW® Detection Kits for Western Blot analysis quickly and clearly reveal bands on the transfer membrane without the need for specialized equipment.

Eventhough chemiluminescent detection is a common method of visualization this involves spending time in a tight dark room, with a timer and a list of exposure times, which are mostly chosen based on educated guesswork.

• A simple solution for real-time results – no film developer, fluorescent reader nor time in the dark room required.
• High sensitivity – quickly visualize your bands with minimal overexposure risk and with no visible background.
• Convenient and easy to use – includes secondary antibody and all reagents necessary to visualize your bands.

For high sensitivity, ease of use, and long-lasting signal, try our new WESTERNVIEW® Detection Kits

• Specific – to bind your protein of interest or primary antibody
• Sensitive – for high detection and low background
• Reliable – for consistent results
• Validated – for Western blot application and stated species

Optimized for Western Transfer – Polyvinylidene Fluoride (PVDF) Membranes

• Sensitive protein detection with low background and very low burn-through
• Provides high surface area for strong hydrophobic interactions (adsorb 50% more protein than nylon or nitrocellulose.)
• Broad solvent compatibility

Protein ladder for use with protein gel electrophoresis

• 3 μL or 5 μL per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively
• Three color protein standard with 13 prestained proteins
• Apply more for thicker (>1.5 mm) or larger gel

Our protein ladder is designed for monitoring protein separation during SDS-polyacrylamide (PA) gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, or add reducing agent before loading.

Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Trisglycine buffer).